RG Microenvironment (Lutzny-Geier)
- 3D-BMSC/Leukemia model
- CAR-T cell functionality
- Mammacarcinoma organoids and metastasis
Staff
- Dr. rer. nat. Gloria Lutzny-Geier, principle investigator
- Franziska von Heydebrand, BTA
- Janin Dingfelder, PhD student
- Jana Lindacher, PhD student
- Kathrina Spießl, MD student
- Anna-Katharina Wilbrand, Master student
- Max Meyer zu Schwabedissen, MD student
- Sarah Handl, MD student
Scientific focus
Our group is interested in the communication of tumor cells with their microenvironment. The tumor microenvironment is essential in the establishment of B-cell lymphomas. Understanding how different signaling pathways get activated through intrinsic signals of the tumor cell itself and extrinsic signals of the microenvironment, is one aim of our studies. Therefore, we investigate how the microenvironment is modulated by tumor cells in 3-dimensional settings and if interference with this modulation can be used as new therapeutic approaches for lymphoma patients.
We also use the principle of the 3D cell culture in solide tumors, like mammacarcinomas. By generating primary organoids, we can characterize the communication and migration of immune cells as well as the metastatic potential of the tumors.
Dr. rer. nat. Gloria Lutzny-Geier
Hartmannstraße 14, Kussmaul-Campus
91052 Erlangen
Phone
+49 9131 85-43169
Current Projects
- Communication of malignant B cells with T cells and mesenchymal stromal cells in the bone marrow niche:
- 3-dimensional in vitro models
- CAR-T cell functionality
- Metabolism of the microenvironment
- Organoids of mammacarcinomas: Metastasis and T cell function
Methods
- Flow cytometry
- ELISA
- Quantitative RT-PCR
- Westernblot
- 3D confocal microscopy
- IHC-IF, H&E staining
- 3D-cell cultures, organoids
Collaborations internal
- Prof. Dr. Metzler (Kinderklinik)
- Prof. Dr. Fasching (Frauenklinik)
- Dr. Tripal (OICE)
Collaborations external
- Prof. Dr. Oostendorp, Klinikum rechts der Isar, TU München
- Prof. Dr. Egle, Cancer Research Institute Salzburg, Österreich
- Dr. Bosch, Leiden, Niederlande
- Prof. Anita Kremer, Zürich
Selected Publications
Handl S, von Heydebrand F, Voelkl S, Oostendorp RAJ, Wilke J, Kremer AN, Mackensen A, Lutzny-Geier G. Immune modulatory effects of Idelalisib in stromal cells of chronic lymphocytic leukemia. Leuk Lymphoma. 2021 Nov;62(11):2679-2689. doi: 10.1080/10428194.2021.1927019. Epub 2021 May 17. PMID: 33999745.
von Heydebrand F, Fuchs M, Kunz M, Voelkl S, Kremer AN, Oostendorp RAJ, Wilke J, Leitges M, Egle A, Mackensen A, Lutzny-Geier G. Protein kinase C-β-dependent changes in the glucose metabolism of bone marrow stromal cells of chronic lymphocytic leukemia. Stem Cells. 2021 Feb 4. doi: 10.1002/stem.3352. Epub ahead of print. PMID: 33539629.
Bernhardt AL, Zeun J, Marecek M, Reimann H, Kretschmann S, Bausenwein J, van der Meijden ED, Karg MM, Haug T, Meintker L, Lutzny-Geier G, Mackensen A, Kremer AN. Influence of DM-sensitivity on immunogenicity of MHC class II restricted antigens. J Immunother Cancer. 2021 Jul;9(7):e002401. doi: 10.1136/jitc-2021-002401. PMID: 34266882; PMCID: PMC8286791.
Mangolini M, Götte F, Moore A, Ammon T, Oelsner M, Lutzny-Geier G, Klein-Hitpass L, Williamson JC, Lehner PJ, Dürig J, Möllmann M, Rásó-Barnett L, Hughes K, Santoro A, Méndez-Ferrer S, Oostendorp RA, Zimber-Strobl U, Peschel C, Hodson DJ, Schmidt-Supprian M,Ringshausen I. Notch2 controls non-autonomous Wnt-signalling in chronic lymphocytic leukaemia. Nature Communications. 2018 Sep 21;9(1):3839. PMCID: PMC6155045
Lutzny G, Kocher T, Rudelius M, Schmidt-Supprian M, Klein-Hitpass L, Finch A, Dürig J, Haferlach T, Seifert M, Wanninger S, Oostendorp R, Ruland J, Leitges M, Kuhnt T, Wagner M, Feuerstacke Y, Peschel C, Egle A, Ringshausen I. Proteinkinase C-β dependent activation of NF-κB in stromal cells is indispensable for the survival of chronic lymphocytic leukemia B-cells in vivo. Cancer Cell. 2013 Jan 14;23(1):77-92. PMCID:PMC3546417